abstract：OBJECTIVE:Third generation oral contraceptives (OC) may increase the risk of cardiovascular events in healthy young women. DESIGN AND METHODS:We assessed the effect of second and third generation OC on CRP, lipids and apolipoproteins in 128 women. RESULTS:CRP was significantly higher in third generation contraceptive...

journal_title：Clinical biochemistry

authors： Krintus M,Sypniewska G,Kuligowska-Prusinska M

更新日期：2010-04-01 00:00:00

abstract：OBJECTIVE:To evaluate clinical value of oxidized lipoprotein(a) [ox-Lp(a)] levels. DESIGN AND METHODS:Ox-Lp(a) were measured by 2 ELISAs using antibodies against ox-Lp(a) [ox-Lp(a)1] or oxidized low-density lipoprotein [ox-Lp(a)2], and studied in 161 acute coronary syndromes (ACS) patients, 114 stable coronary artery ...

journal_title：Clinical biochemistry

authors： Wang JJ,Han AZ,Meng Y,Gong JB,Zhang CN,Li K,Liu YX

更新日期：2010-04-01 00:00:00

abstract：OBJECTIVES:To report the clinical and laboratory characteristics of urea cycle disorder (UCD) patients at a tertiary care center in a developing country. DESIGN AND METHODS:Retrospective study of clinical and laboratory data of UCD patients. RESULTS:Thirty-seven UCD patients were studied, 31 symptomatic (high risk) p...

journal_title：Clinical biochemistry

authors： Ibarra-González I,Fernández-Lainez C,Vela-Amieva M

更新日期：2010-03-01 00:00:00

abstract：OBJECTIVE:To characterize the lipid-related atherogenic risk factors in iron deficiency anaemia (IDA) patients. DESIGN AND METHODS:Twenty IDA women were compared to healthy age-matched controls. Lipoprotein profile, cholesteryl ester transfer protein (CETP), paraoxonase (PON) 1 and lipoprotein-associated phospholipase...

journal_title：Clinical biochemistry

authors： Meroño T,Sorroche P,Gómez Rosso LA,Casañas L,Boero LE,Arbelbide JA,Brites FD

更新日期：2010-03-01 00:00:00

abstract：OBJECTIVES:Although numerous recent studies have focused on analyses of microRNA expression profiles in cancer cells, the expression patterns of the enzymes responsible for the generation of miRNAs remains largely unexplored. The purpose of this study was to investigate whether Dicer mRNA expression is altered during p...

journal_title：Clinical biochemistry

authors： Pampalakis G,Diamandis EP,Katsaros D,Sotiropoulou G

更新日期：2010-02-01 00:00:00

abstract：OBJECTIVES:To test the hypothesis that impaired expression of the thiosulfate sulfurtransferase rhodanese is associated with oxidative stress and may predict mortality in hemodialysis patients. DESIGN AND METHODS:Sixty-two hemodialysis patients were investigated to determine protein and mRNA expression of rhodanese in...

journal_title：Clinical biochemistry

authors： Krueger K,Koch K,Jühling A,Tepel M,Scholze A

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVES:Recurrent miscarriage (RM) is a challenging medical problem because of its unknown pathogenesis and etiology in most of the cases. Recent studies suggest the role of persistent environmental pollutants such as organochlorine pesticides (OCPs) in the etiology of RM. The present study was conducted to investig...

journal_title：Clinical biochemistry

authors： Pathak R,Mustafa M,Ahmed RS,Tripathi AK,Guleria K,Banerjee BD

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVES:: To test if circulating forms of the soluble urokinase-type plasminogen activator receptor (suPAR) are potential biomarkers of plaque vulnerability. DESIGN AND METHODS:: Plasma concentrations of suPAR(I-III), suPAR(II-III) and uPAR(I) were measured by time-resolved fluorescence immunoassays in Caucasian pa...

journal_title：Clinical biochemistry

authors： Olson FJ,Thurison T,Ryndel M,Høyer-Hansen G,Fagerberg B

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVE:We seek to identify the differentially expressed miRNAs in the clear cell subtype (ccRCC) of kidney cancer. DESIGN AND METHODS:We performed a miRNA microarray analysis to compare the miRNA expression levels between ccRCC tissues and their normal counterpart. The top dysregulated miRNAs were validated by quan...

journal_title：Clinical biochemistry

authors： Chow TF,Youssef YM,Lianidou E,Romaschin AD,Honey RJ,Stewart R,Pace KT,Yousef GM

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVES:Homologous recombination is a frequent phenomenon in multigene families and as such it occurs several times in both the alpha- and beta-like globin gene families. In numerous occasions, genetic recombination has been previously implicated as a major mechanism that drives mutagenesis in the human globin gene ...

journal_title：Clinical biochemistry

authors： Borg J,Georgitsi M,Aleporou-Marinou V,Kollia P,Patrinos GP

更新日期：2009-12-01 00:00:00

abstract：BACKGROUND:Genetic variations of UDP-glucuronyltransferase 1A1 (UGT1A1) influence the concentration of serum bilirubin. We investigated the association of four common polymorphisms including UGT1A1-53(TA)(n), and common haplotypes of the UGT1A1 gene with bilirubin levels in 218 Caucasian volunteers. METHODS:Total bili...

journal_title：Clinical biochemistry

authors： Borucki K,Weikert C,Fisher E,Jakubiczka S,Luley C,Westphal S,Dierkes J

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVES:This study was undertaken to investigate the impact of the Pro12Ala (rs1801282) polymorphism of the peroxisome proliferator-activated receptor gamma-2 (PPARgamma-2) gene on obesity or body mass index (BMI) and plasma leptin, insulin, adiponectin and lipid levels in a sample of the Tunisian population. DESIG...

journal_title：Clinical biochemistry

authors： Ben Ali S,Ben Yahia F,Sediri Y,Kallel A,Ftouhi B,Feki M,Elasmi M,Haj-Taieb S,Souheil O,Sanhagi H,Slimane H,Jemaa R,Kaabachi N

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVES:The aim of the study was to evaluate the concentration of interleukin 12 (IL-12), the activity of phospholipase A(2) (PLA(2)), and platelet-activating factor acetylhydrolase (PAF-AH) in type 1 diabetes (DM1) patients treated with intensive insulin therapy. DESIGN AND METHODS:Studied parameters were measured...

journal_title：Clinical biochemistry

authors： Wegner M,Araszkiewicz A,Pioruńska-Mikołajczak A,Zozulińska-Ziółkiewicz D,Wierusz-Wysocka B,Pioruńska-Stolzmann M

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVES:Preeclampsia, the most common pregnancy-associated pathological syndrome, is accompanied by significant remodelling of the extracellular matrix and alteration in lipid composition of the umbilical cord artery (UCA). DESIGN AND METHODS:We evaluate the sphingolipid composition of UCA and its alteration in pre...

journal_title：Clinical biochemistry

authors： Romanowicz L,Bańkowski E

更新日期：2009-11-01 00:00:00

abstract：OBJECTIVE:Evaluation of serum beta-2-microglobulin (beta2M) in an automated analyzer. DESIGN AND METHODS:The DakoCytomation beta2M kit is an antibody based reagent intended for quantitative determination of beta2M in serum and plasma by rate nephelometry. RESULTS:The limit of blank is 0.16 mg/L. The method is linear ...

journal_title：Clinical biochemistry

authors： De BK,Jimenez E,De S

更新日期：2009-10-01 00:00:00

abstract：OBJECTIVES:To compare HPLC methods with short and long elution times for HbA1c measurement in blood. METHODS:Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). RESULTS:Passing-Bablok regression equations were: Y=0.17+0.96X; Y=-0.39+1.01X; Y=-0.40+1.0X and the means...

journal_title：Clinical biochemistry

authors： Leon-Justel A,Santotoribio JD,Dominguez-Pascual I,Delgado AL,Macias C,Herrera MT,Leal A,Mangas MA,Acosta D,Guerrero JM

更新日期：2009-10-01 00:00:00

abstract：:Immunoturbidimetric assays for specific proteins are available on "open system" clinical chemistry analyzers. The analytical performance of nine immunoturbidimetric specific protein assays (C3, C4, CRP, Haptoglobin, IgA, IgG, IgM, RF, and Transferrin) was compared to immunonephelometry. Testing was performed on the Ab...

journal_title：Clinical biochemistry

authors： Mali B,Armbruster D,Serediak E,Ottenbreit T

更新日期：2009-10-01 00:00:00

abstract：OBJECTIVES:To investigate the activities of the main antioxidative enzymes and oxidative stress in women with depressive disorder (DD). METHODS:In 35 drug-naive women with DD and 35 age matched healthy women enzymes superoxide dismutase (CuZnSOD), catalase (CAT), glutathione peroxidase (GPX1), glutathione reductase (G...

journal_title：Clinical biochemistry

authors： Kodydková J,Vávrová L,Zeman M,Jirák R,Macásek J,Stanková B,Tvrzická E,Zák A

更新日期：2009-09-01 00:00:00

abstract：OBJECTIVES:Oxidative stress plays a role in the pathogenesis in patients with HCV infection. The objective of this study was to evaluate oxidant and antioxidant biomarkers in patients with HCV. DESIGN AND METHODS:Serum malonaldehyde (MDA) and nitric oxide (NO) levels and the activities of myeloperoxidase (MPO), aryles...

journal_title：Clinical biochemistry

authors： Ali EM,Shehata HH,Ali-Labib R,Esmail Zahra LM

更新日期：2009-09-01 00:00:00

abstract：OBJECTIVES:This study has been conducted to develop a proper and relatively simple method for purification of three major forms of beta-hCG from urine to use as clinical calibrators and also to produce anti-beta-hCG polyclonal antibodies against these purified forms. DESIGN AND METHODS:Major beta-hCG forms from urine ...

journal_title：Clinical biochemistry

authors： Khademi F,Hamzehee K,Mostafaie A,Hajihossaini R

更新日期：2009-09-01 00:00:00

abstract：OBJECTIVES:Oxidative stress increases postmenopausally, an effect attributed to aging. Increase in body weight generally accompanies menopause. Obesity per se also seems to increase oxidative stress. The question is whether postmenopausal increase in oxidative stress is related to body weight, or is only a function of ...

journal_title：Clinical biochemistry

authors： Mittal PC,Kant R

更新日期：2009-07-01 00:00:00

abstract：OBJECTIVES:To assess the medical applicability of CpG methylation as molecular markers for cancer diagnosis, we established a new system to determine DNA methylation based on TaqMan PCR combined with a methyl-binding-domain polypeptide 2. DESIGN AND METHODS:We evaluated the diagnostic applicability of this approach by...

journal_title：Clinical biochemistry

authors： Kimura N,Moribe T,Iizuka N,Miura T,Tamatsukuri S,Ishitsuka H,Hamamoto Y,Oka M

更新日期：2009-07-01 00:00:00

abstract：OBJECTIVE:Can biochip arrays identify which individuals with metastatic disease will respond to an anti-metastatic agent? DESIGN AND METHODS:Cytokine and cell adhesion arrays (Randox Ltd) were measured over 1 month in 9 research participants receiving CTCE-9908 in a Phase I/II study. RESULTS:Research participants wit...

journal_title：Clinical biochemistry

authors： Kavsak PA,Henderson M,Moretto P,Hirte H,Evans K,Wong D,Korz W,Hotte SJ

更新日期：2009-07-01 00:00:00

abstract：OBJECTIVE:To determine Retinol Binding Protein 4 (RBP4) levels in patients with Familial Partial Lipodystrophy (FPLD). METHODS:Ten patients with FPLD and a control group (9 patients) were selected to participate in the study. RESULTS:RBP4-log levels were lower in patients with FPLD in comparison to control group (1.5...

journal_title：Clinical biochemistry

authors： Godoy-Matos AF,Moreira RO,MacDowell R,Bendet I,Mory PB,Moises RS

更新日期：2009-07-01 00:00:00

abstract：OBJECTIVES:Despite the evidence that both homocysteine and cysteine are important risk factors for vascular disease and atherosclerosis no information are reported about their effective amount in plaque and on the relationship with the other low molecular weight thiols. DESIGN AND METHODS:We used capillary electrophor...

journal_title：Clinical biochemistry

authors： Zinellu A,Lepedda A Jr,Sotgia S,Zinellu E,Scanu B,Turrini F,Spirito R,Deiana L,Formato M,Carru C

更新日期：2009-06-01 00:00:00

abstract：OBJECTIVES:We investigated the stability of 36 analytes related to clinical chemistry in a controlled storage study. DESIGN AND METHODS:Blood was collected from 11 subjects and was maintained for 45 min, 2.5 h, 5 h, or 24 h after phlebotomy before centrifugation. RESULTS:Statistically significant changes were observe...

journal_title：Clinical biochemistry

authors： Zwart SR,Wolf M,Rogers A,Rodgers S,Gillman PL,Hitchcox K,Ericson KL,Smith SM

更新日期：2009-06-01 00:00:00

abstract：OBJECTIVES:To measure serum cholinesterase (SCHE) with an integration strategy. DESIGN AND METHODS:At 54.0 micromol/L butyrylthiolcholine, SCHE initial rates were calculated with 50.0 micromol/L butyrylthiolcholine and maximal rates via an improved integrated method if substrate consumptions within 5.0 min were over 6...

journal_title：Clinical biochemistry

authors： Liao F,Yang D,Tang J,Yang X,Liu B,Zhao Y,Zhao L,Liao H,Yu M

更新日期：2009-06-01 00:00:00

abstract：OBJECTIVES:To investigate whether the STK15 mRNA expression correlates with clinicopathologic features and the prognosis of HCC patients. DESIGN AND METHODS:Three hepatoma cell lines, two normal liver epithelial cell lines, hepatoma tissues, adjacent tumor tissues and normal liver tissues were obtained from 46 HCC pat...

journal_title：Clinical biochemistry

authors： Wang R,Wang JH,Chu XY,Geng HC,Chen LB

更新日期：2009-05-01 00:00:00

abstract：OBJECTIVES:We aimed to compare the individuality (within subject consistency) of plasma and urine betaine and N,N-dimethylglycine. DESIGN AND METHODS:In two separate groups of 8 males (ages 19 to 40), plasma (10) and urine (6) samples were collected either over a single day or over an 8 week period. The individuality ...

journal_title：Clinical biochemistry

authors： Lever M,Atkinson W,Slow S,Chambers ST,George PM

更新日期：2009-05-01 00:00:00

abstract：OBJECTIVE:This study was conducted to investigate the potential role of intravascular oxidative stress in inexplicable episodes of coronary artery spasm (CAS) during coronary angiography (CAG). DESIGN AND METHODS:Serial patients with stable angina scheduled for routine CAG were prospectively evaluated. Patients with c...

journal_title：Clinical biochemistry

authors： Leu HB,Lin WT,Lin CP,Wu TC,Lin SJ,Chen JW

更新日期：2009-05-01 00:00:00

abstract：OBJECTIVES:Elevated advanced glycation endproducts (AGEs) are implicated in diabetic complications. Methylglyoxal-derived hydroimidazolone (MG-H) is one of the most abundant AGEs in vivo. Our objective was to develop a time-saving, specific method to measure free MG-H in plasma and determine its levels in complication-...

journal_title：Clinical biochemistry

authors： Han Y,Randell E,Vasdev S,Gill V,Curran M,Newhook LA,Grant M,Hagerty D,Schneider C

更新日期：2009-05-01 00:00:00

abstract：OBJECTIVES:Patients with Ehlers-Danlos syndrome were described to contain reduced activities of beta4-galactosyltransferase-7 (beta4Gal-T7). Therefore, measurement of beta4Gal-T7 activity can help to characterize defects in proteoglycan biosynthesis in patients with connective tissue diseases. DESIGN AND METHODS:We de...

journal_title：Clinical biochemistry

authors： Kuhn J,Kleesiek K,Götting C

更新日期：2009-04-01 00:00:00

abstract：OBJECTIVES:High-resolution melting analysis has been validated for screening several genes of clinical significance. We evaluated whether it would be useful for detecting mutations in the LDL receptor gene (LDLR). DESIGN AND METHODS:We analysed 60 samples heterozygous for different LDLR sequence variants, and compared...

journal_title：Clinical biochemistry

authors： Laurie AD,George PM

更新日期：2009-04-01 00:00:00

abstract：AIM:Medical laboratories are the key partners in patient safety. Laboratory results influence 70% of medical diagnoses. Quality of laboratory service is the major factor which directly affects the quality of health care. The clinical laboratory as a whole has to provide the best patient care promoting excellence. METH...

journal_title：Clinical biochemistry

authors： Guzel O,Guner EI

更新日期：2009-03-01 00:00:00

abstract：UNLABELLED:Experimental studies confirmed that reactive oxygen species increase endothelin-1 (ET-1) synthesis, and modulate ET-1 signaling pathway resulting in vasoconstriction and vascular remodeling. The aim of this study was to evaluate the relationship between plasma ET-1 concentration and antioxidant status in pat...

journal_title：Clinical biochemistry

authors： Skalska AB,Pietrzycka A,Stepniewski M

更新日期：2009-03-01 00:00:00

abstract：OBJECTIVES:To investigate the association of parental hyperhomocysteinemia, C677T Methylene tetrahydrofolate reductase (MTHFR) polymorphism and DNA damage with recurrent pregnancy loss (RPL). DESIGN AND METHODS:A case-control study. Reverse phase HPLC, PCR-RFLP and Cytokinesis blocked micronuclei assay were used to as...

journal_title：Clinical biochemistry

authors： Govindaiah V,Naushad SM,Prabhakara K,Krishna PC,Radha Rama Devi A

更新日期：2009-03-01 00:00:00

abstract：BACKGROUND:Currently, 20 IU/L is the accepted sensitivity of over-the-counter (OTC) hCG pregnancy tests, and the only sensitivity permitted for point of care (POC) tests. These sensitivity limitations cause significant numbers of false-negative tests, which mislead women and cause them to avoid precautions needed to av...

journal_title：Clinical biochemistry

authors： Cole LA,Ladner DG

更新日期：2009-02-01 00:00:00

abstract：:1,5-anhydroglucitol (AG) is a nonmetabolizable glucose analogue found in plasma due to ingestion. The normal steady-state concentration can be dramatically decreased by inhibition of tubular reabsorption during periods of hyperglycemia. For this reason, monitoring of AG has been plausibly advocated for detection of pe...

journal_title：Clinical biochemistry

authors： Nerby CL,Stickle DF

更新日期：2009-02-01 00:00:00

abstract：OBJECTIVE:To confirm that the naproxen metabolite O-desmethylnaproxen interferes with the Jendrassik and Grof method for total bilirubin and was a possible cause of spurious hyperbilirubinemia in a case of naproxen overdose. DESIGN AND METHODS:Plasma was spiked with naproxen or O-desmethylnaproxen before total and dir...

journal_title：Clinical biochemistry

authors： Al Riyami N,Zimmerman AC,Rosenberg FM,Holmes DT

更新日期：2009-01-01 00:00:00

abstract：OBJECTIVES:To evaluate platelet activating factor (PAF) levels, its metabolic enzymes activity and its associations with other inflammatory markers in heart failure (HF) patients. DESIGN AND METHODS:PAF, and two of its key biosynthetic enzymes [lyso-PAF acetyltransferase (lyso-PAF-AT) and DTT-insensitive CDP-choline:1...

journal_title：Clinical biochemistry

authors： Detopoulou P,Nomikos T,Fragopoulou E,Antonopoulou S,Kotroyiannis I,Vassiliadou C,Panagiotakos DB,Chrysohoou C,Pitsavos C,Stefanadis C

更新日期：2009-01-01 00:00:00